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血浆D-二聚体检测及其临床应用

Measurement of plasma D-dimer and its clinical application

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【作者】 黄尉国王鸿利张颖琪朱立红李耀辉王学锋程寅琳邵慧珍张鲁葛维成

【Author】 Huang Weiguo;Wang Hongli;Zhang Yinqi,et al.(Shanghai No7 People’s Hospital,Shanghai 200137)

【机构】 上海市第七人民医院上海第二医科大学瑞金医院上海市捷门生物技术公司

【摘要】 利用自制单克隆抗体,建立酶联免疫吸附试验(ELISA)双抗体夹心法检测血浆D-二聚体含量,灵敏度达8ng/ml。检测30例正常青壮年为0.13±0.03mg/L,13例健康老年人为0.44±0.18mg/L二组比较差异有显著性意义(P<0.01)。检测50例肝脏疾病、28例急性粒细胞白血病、35例急性心肌梗塞、60例脑血管疾病和22例外科手术的患者,结果血浆D-二聚体水平均较正常对照组升高(P<0.001);同时急性心肌梗塞组和脑血管疾病组也较健康老年人组为高(P<0.01,P<0.001)。表明D-二聚体检测是一项鉴别原发性纤溶和继发性纤溶以及监测溶栓治疗的有价值的指标。

【Abstract】 he anthns measured the concentration of D-dimer in plasma by a sandwich-ELISA assay with two self-made McAbs.Its detection sensitivity was able to reach 8ng/ml.The average concentration of D-dimer in 30 normal adults was 0.13±0.03 mg/L, while that of D-dimer in 13 health olders was 0.44±0.18 mg/L,with a significant discrepancy between this two groups(P< 0.01).The investigation on 50 patients with liver diseases, 28 patients with acute graiiulocytic leukemia, 35 patients with acute my-ocardial infarction (AMI),60 patients with cerebrovascular diseases(CVD) and 22 patents after their surgical operations showed that the D-dimer levels in all of these persons were higher than those in con-trol group (P<0. 001),and that the D-dimer levels in AMI and CVD were higher than that in health older group(P<0.01,P<0.001 respectively)。

【关键词】 纤维蛋白溶解; D-二聚体;

【Key words】 Fibrinolysis D-dimer;

【分类号】R313;R331.16;R457.14;

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