CHKD期刊全文数据库

节点文献

骨髓间充质干细胞分化为血管内皮细胞的实验研究

An experimental study on the differentiation of bone marrow mesenchymal stem cells into vascular endothelial cells

  • 推荐 CAJ下载
  • PDF下载
  • 不支持迅雷等下载工具,请取消加速工具后下载。

【作者】 方利君付小兵孙同柱李建福程飚杨银辉王玉新

【Author】 FANG Li-jun,FU XIAO-bing,SUN TONG-zhu,LI Jian-fu,CHENG Biao,YANG Yin-hui,WANG Yu-xin.Department of Stomatology,The Second Affiliated Clinical College,Chinese Medical University,Shen Yang 110003,Liao Ning Province;Burns Institute, 304th Hospital,Beijing 100037 P.R.China

【机构】 中国医科大学附属第二临床学院口腔科解放军第三○四医院创伤外科研究室解放军第三○四医院中国医科大学附属第一临床学院口腔颌面外科 110003沈阳100037北京100037北京

【摘要】 目的 探讨骨髓间充质干细胞 (MSCs)分化为肉芽组织中血管内皮细胞的可能性 ,及其参与创面修复的可能机制。 方法 抽取小型香猪的骨髓 ,经密度梯度离心分离、纯化MSCs,体外培养扩增后 ,应用溴脱氧尿嘧啶 (BrdU)标记技术标记细胞。于已抽取骨髓的小型香猪背部制作全层皮肤缺损创面 ,即刻以纤维蛋白胶为载体 ,将已标记的MSC回植到供体动物创面上。术后 2、4、6、8、12周切取创面组织 ,行BrdU和因子Ⅷ (FⅧ )免疫组织化学染色 ,进行对比观察。 结果 BrdU阳性的MSCs多聚集在创面肉芽组织中的小血管周围 ,且有个别血管内皮细胞也呈现BrdU阳性。部分BrdU阳性的MSCs胞浆中亦有FⅧ表达。 结论 创面愈合过程中 ,MSCs与肉芽组织中小血管的形成密切相关。在创面微环境下 ,MSCs可分化为血管内皮细胞 ,并参与创面修复。

【Abstract】 Objective To investigate the feasibility of differentiation of bone marrow mesenchymal stem cells (MSCs) into vascular endothelial cells and the mechanism of its involvement in wound healing. Methods Porcine MSCs were harvested from porcine marrow, and they were isolated and purified by density gradient centrifugation. After being cultured and amplified in vitro, the MSCs were labelled with BrdU (5-bromodeoxy-uridine). Full skin loss wound was created on the back of the mini-swine whose bone marrow was obtained. The labelled MSCs with fibrin glue as the vector were regrafted back to the donor animal wound. The wound tissue specimens were harvested at 2, 4, 6, 8 and 12 post-operation weeks and were immunohistochemically stained by BrdU and factor Ⅷ (FⅧ) for comparative study. Results Most BrdU positive cells aggregated around small blood vessels in the granulation tissue of the wounds. Only individual vascular endothelial cells were BrdU positive. There was FⅧ expression in the cytoplasm of BrdU positive cells . Conclusion MSCs were closely correlated with the formation of small blood vessels in granulation tissue during wound healing process. The porcine MSCs possessed the potential to differentiate into vascular entoehelial cells and to participate in wound healing under the micro-enviroment of the wound. [

【关键词】 干细胞; 骨髓; 内皮,血管; 创面修复;

【Key words】 Stem Cells; Bone Marrow; Vascular endothelial cells; Wound repair;

【分类号】R329.24;R329.51;R322.12;

【基金】 国家重点基础研究发展规划资助项目 (G19990 5 42 0 4);国家自然科学基金面上项目 ( 30 170 96 6 );国家自然科学基金重点项目 ( 30 2 30 370 )

  • 【下载频次】794
节点文献中: